Author's response to reviews Title: DNA methylation analysis reveals distinct methylation signatures in pediatric germ cell tumors Authors:

Author's response to reviews Title: Comprehensive profiling of DNA methylation in colorectal cancer reveals subgroups with distinct clinicopathological and molecular features Authors:

Removed underlining from manuscript Updated reference 25 to include full details Changed " Figure " to " Fig. " where present Added " in the GoldenGate Methylation Array data " to the end of paragraph 2 on page 10 to address the remaining comment from Reviewer 2.

The role and importance of DNA methylation in spermatogenesis process

Background: DNA methylation is one of the epigenetic marks that are created by de novo DNA methylation and be maintained through cell division. This process is catalyzed by DNA methyltransferases. DNA methylation establishment in germ line is important, since they have the potential to regulate gene expression in offspring and improper DNA methylation patterns in germ lines has serious conseque...

Author's response to reviews Title: Diet Folate, DNA Methylation and Genetic Polymorphisms of MTHFR C677T in Association with the Prognosis of Esophageal Squamous Cell Carcinoma Authors:

O6-Methylguanine-DNA Methyltransferase and ATP-Binding Cassette Membrane Transporter G2 Promotor Methylation: Can Predict the Response to Chemotherapy in Advanced Breast Cancer?

Background: ATP-binding cassette membrane transporter G2 (ABCG2) gene is one of transporter family and well characterized for their association with chemoresistance. Promoter methylation is a mechanism for regulation of gene expression. O6-Methyl guanine DNA methyl transferase (MGMT) gene plays a fundamental role in DNA repair. MGMT has the ability to remove alkyl adducts from DNA at the O6 pos...

I-50: Embryo Loss Due to Epigenetic Anomaliesin the Male Germ Line: Role of Estrogen

Background: To investigate if aberrant methylation and expression of imprinted genes of the Igf2-H19 locus in the spermatozoa and embryos could be a paternal epigenetic factor involved in early embryo loss To elucidate the role of estrogen in acquisition of the imprinting at the Igf2-H19 locus during spermatogenesis Materials and Methods: Adult male rats of Holtzman strain were administered tam...